Effect of Alfalfa-Derived Biochar on Anaerobic Digestion of Dairy Manure

Biochemical methane potential (BMP) tests were conducted for investigating the effects of alfalfa-derived biochar (AF-BC) on anaerobic digestion (AD) dairy manure under various loading AF-BC (0–10 g/L). BMP performed at mesophilic temperature (37 °C) with addition AF-BC. Biogas and volumes concentrations, water quality parameters (i.e., COD (chemical oxygen demand)), volatile fatty acids (VFAs) measured during AD process. The 1 5 g/L increased biogas yields by 15.51% 26.09% 14.61% 26.88% compared control without Additionally, (1–10 g/L) decreased lag phase 7.14–22.45% CO2 content 13.60–32.48%, while increasing removal efficiency 19.19–35.94% in manure. Moreover, also total VFAs acetic acid concentrations increase performance was mainly owing to improvement buffering ability system direct interspecies electron transfer (DIET) among microorganisms resulting from In contrast, 10 did not show any obvious manure, possibly because toxic excessive toward microorganisms. Therefore, this study supported practical feasibility AF-BC-enhanced